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Protein A Antibody Purification Kit

The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
Catalog #: K974
$295.00

Product Details

Cat # +Size K974-10
Size 10 purifications
Kit Summary The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
Applications Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
Features & Benefits • Ready-to-use pre-packed columns of 5 ml bead volume in 20% Ethanol.• HIGH BINDING CAPACITY: Binding of IgG ≥30 mg human or rabbit IgG/ml Protein A-Agarose• USAGE: Reusable for up to 10 times without significant loss of binding capacity.
Kit Components • Hi-Bind Protein Column
• Binding Buffer (10X)
• Elution Buffer (10X)
• Neutralization Buffer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins. BioVision’s Protein A (Cat. No. 6500 and 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions. Hi-BindTM Protein A-Agarose beads (Cat. No. 6520) for purification of IgG contain covalently coupled recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein purification. BioVision’s Protein A Antibody purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 350 mg of IgG. The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.


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What is the maximum sample volume I can use on the column at once?
The maximum sample volume that can be used at once is 4 ml. If the sample is more than 4 ml (for eg: 12 ml serum), then load the column 3 times. Between each use, regenerate the column as follows: Wash the column with 10 ml Elution Buffer, followed by 5 ml distilled water twice and then 5 ml binding buffer twice.
  i. Do NOT carry out the storage procedure between the 3 loading times – i.e, DO NOT add 5 ml 20% ethanol into the column, drain out 3 ml and store at 4°C (last part of Step VIII-7) in between the 3 loading times.
  ii. Storage procedure should be performed only at the end when the column needs to be stored for future use.