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Protein A-Agarose

based on 1 citations in multiple journalsProtein A-Agarose14.1 4
Reusable Protein A conjugated agarose beads with ≥20 mg/ml binding capacity & 2.89 ml/min flow rate for antibody purification from multiple sources
Catalog #: 6526

In stock

SKU-Size Size Price Qty
6526-1 1 ml
$95.00
6526-5 5 ml
$340.00
6526-25 25 ml
$1,035.00
6526-100 100 ml
$2,675.00
More Sizes Get Quote

Product Details

Highlights • CONTENTS
- Supplied as a 50% slurry in 20 % Ethanol/H2O.
- HIGH BINDING CAPACITY: Binding of IgG ≥ 20 mg human or rabbit IgG/ml Protein A-Agarose.
- MINIMAL LEACHING OF THE LIGAND.
- FLOW RATE TESTED*: 2.89 ml/min.
*Test condition: Calculations based on the time required to pass 18 ml of water through 2 ml settled beads (column diameter 1.5 cm).
- USAGE: Reusable for up to 10 times without significant loss of binding capacity.
• APPLICATIONS
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein affinity purification methods. BioVision Protein A (Cat. # 6500, Cat. # 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A than the other regular Protein-A Agarose on the market. The IgG binding capacity of Protein A-Agarose is ≥ 20 mg human or rabbit IgG per ml of wet beads. Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation.


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Sen et al., Localization and Function of Pals1-associated Tight Junction Protein in Drosophila Is Regulated by Two Distinct Apical Complexes. J. Biol. Chem., May 2015; 290: 13224 - 13233.
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