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Protein A Agarose (Endotoxin-free)

Reusable, Endotoxin free Protein A conjugated Agarose beads with ≥15 mg/ml binding capacity & 0.85 cm/min flow rate for antibody purification from multiple sources
Catalog #: M1320

In stock

SKU-Size Size Price Qty
M1320-1 1 ml
$195.00
M1320-5 5 ml
$485.00
M1320-25 25 ml
$1,595.00
More Sizes Get Quote

Product Details

Highlights • CONTENTS- Supplied as a 50% slurry in 20 % Ethanol/H2O
-ENDOTOXIN FREE
- HIGH BINDING CAPACITY: Binding of IgG ≥15 mg human or rabbit IgG/ml Protein A-Agarose.
- MINIMAL LEACHING OF THE LIGAND.
- FLOW RATE TESTED*: 0.85 cm/min.
*Test condition: Linear flow rate determined in 2 ml column with internal diameter of 1.5 cm.
• APPLICATIONS
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for
binding antigen.
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Endo toxin free Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads under proprietary endotoxin-free conditions. BioVision’s Protein A (Cat. No. 6500, Cat. No. 6500B) is a genetically engineered protein containing five IgG-binding regions of native Protein A. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. Protein A-Agarose beads display high chemical & physical stability as well as high flow rate, hydrophilicity & high gel strength. It can be used for IgG purification and immunoprecipitation. Protein A-Agarose beads are prepared by covalently coupling recombinant Protein A to 6% cross-linked Agarose beads. The coupling technique is optimized to give a higher binding capacity for IgG & minimum leaching of recombinant Protein A. The IgG binding capacity of Protein A-Agarose is ≥ 15 mg human or rabbit IgG per ml of settled beads. Our Endotoxin-free Protein A-Agarose also shows ability of reducing/removing certain amount of endotoxin from serum or ascites samples.


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