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RT PCR Master Mixes, Kits and Rtases

BioVision offers a wide range of RT PCR enzyme products including RT PCR Kits, cDNA Synthesis Kits and Supermixes, RT PCR Master Mixes, QRT PCR Kits and Reverse Transcriptases (RTases).
cDNA Synthesis Kit contains all the materials required for first-strand cDNA synthesis, with the choice of using either Oligo (dT) and/or Random Primers. The Oligo (dT) anneals selectively to the poly (A) tail of mRNAs. Random Primers are oligonucleotides that anneal at non-specific sites of RNA templates. Therefore, all forms of RNA can be used in cDNA synthesis. They do not require the presence of poly (A) and can be used for the transcription of mRNA 5’-end regions. cDNA Synthesis Kit can be used for synthesizing cDNA from ssRNA, DNA primer extension, sequencing dsDNA, constructing cDNA library, producing template for use in RT PCR, labelling 3'-end of duplex DNA via end-filling reactions, generating probes for hybridization etc

The cDNA Synthesis Supermix is a proprietary mixture of all the materials required for first strand cDNA synthesis in a 2X concentration. A balanced amount of Oligo (dT)and Random Primers is included in the 2X Reaction Mix. The cDNA Synthesis Supermix can be used for generation of templates for use in RT PCR and QRT PCR, cDNA synthesis from ssRNA, cDNA library construction, Generation of probes for hybridization, DNA primer extension etc.

Reverse Transcriptases (RTases) are used for in vitro first strand cDNA synthesis using RNA as the starting template. Reverse transcription of RNA is often required for PCR, real time PCR (QPCR), and gene cloning. Two different RTases (Evo™ and Novo™ Reverse Transcriptase) are available from BioVision, both differ in operational temperatures used in the first-strand cDNA synthesis. BioVision’s RTases are the best in quality, with guaranteed functionality for any RNA template. Furthermore, both the RTases contain an additional fidelity enhancing subunit, thereby drastically reducing the error rate in reverse transcription. This unique feature allows BioVision’s RTases to outperform most other RTases on the market. RTases can be used for synthesizing cDNA from a ssRNA, DNA primer extension, sequencing dsDNA, constructing cDNA library, constructing libraries for serial analysis for gene expression (SAGE), synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE), producing template for use in RT-PCR or real-time RT PCR, labelling 3'-end of duplex DNA via end-filling reactions, generating probes for hybridization etc.

Evo™ Reverse Transcriptase (Evo™ RTase) is an optimized mutational derivative of native RTase enzyme, the Evo™ RTase represents the best-performing RTase on the market. This enzyme catalyzes the synthesis of complementary DNA strands from single-stranded RNA and DNA templates. Due to a series of mutations introduced within the RNase H domain of this enzyme, there is no detectable RNase H activity associated with the enzyme. The lack of RNase H activity helps to eliminate RNA degradation during first-strand cDNA synthesis, resulting in better yield and length of cDNA synthesized.

Novo™ Reverse Transcriptase (Novo™ RTase) is a novel recombinant RTase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. Novo™ RTase is engineered to perform under high temperatures (50°C - 55°C) which facilitate the elimination of secondary structures associated with GC-rich RNA templates. Due to this unique feature, Novo™ RTase can synthesize full length cDNA libraries from RNA templates up to 15 kb in length. In addition, Novo™ RTase has outstanding proofreading ability, thus it can be utilized for whole genome sequencing.

One Step RT PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification in a single reaction tube. Specifically, the One Step RT PCR Kit combines Evo™ RTase and Distant™ DNA Polymerase in a convenient format for highly sensitive and specific RT PCR with any RNA template. Our proprietary RT PCR Buffer contains stabilizers and enhancers that optimize the two reactions into a “single step”. Together with our specially formulated RT PCR Buffer, this One Step RT PCR Kit offers end users an efficient, easy to use, and reliable alternative to conventional “twostep” sequential RT PCR. One Step RT PCR Kit can be used for gene-expression analysis, transcription analysis, gene cloning, Multiplex RT-PCR, high throughput applications, virus detection and quantification etc.

Two Step RT PCR kits provide all the necessary reagents required for reverse transcription and PCR amplification in two separate steps. The kit offers the flexibility of optimizing different RT PCR applications with the choice of either Evo™ RTase or Novo™ RTase. The Novo™ RTase makes it possible to efficiently synthesize first-strand cDNA from RNA templates with secondary structures and high GC content. Two Step RT PCR Kit can be used for routine RT-PCR amplification of RNA templates, reliable RT-PCR based detection of gene expression etc.

Key Features:

  • Convenient, Easy-To-Use and Time Saving Reaction Set Up (RT PCR Kits suitable for complex RNA templates (secondary structures and high GC content))
  • Highly Efficient, Specific, Excellent cDNA yield, High Reproducibility and Robust PCR performance
  • Streamlined Protocol Suitable for High-Throughput Applications
  • Minimal Effort and Maximum Ease: First strand cDNA synthesis with minimal effort and maximum ease even from low-abundance transcripts using BioVision’s cDNA Synthesis Supermix
  • Maximal Flexibility in Priming – oligo(dT), random primers or gene-specific primers using BioVision’s cDNA synthesis Kits
  • Convenient, Sensitive, Reliable, Superb Signal-To-Noise Ratio (in real-time RNA quantification in one step using QRT Kits)


Specifications
Description
 One or Two Step RT PCR Kits
 Two Step RT Kits including Evo™ or Novo™ RTase
 First Strand cDNA Synthesis Kits
 First-Strand cDNA Synthesis Kit including Evo™ or Novo™ RTase
 First Strand cDNA Synthesis Supermixes
 First Strand cDNA Synthesis Supermixes including Evo™ or Novo™ RTase
 5X All-In-One RT Mastermixes
 5X All-In-One RT Mastermix with either Genomic DNA Removal Kit or RNA extraction from cells.
 Reverse Transcriptases
 An optimized mutational derivative of the original Reverse Transcriptase and A novel recombinant reverse transcriptase representing the best-performing RTase on the market
 One Step QRT PCR Kits
 A complete QPCR system containing all necessary reagents for both reverse transcription and PCR amplification to occur in a single QPCR reaction tube

RT PCR Master Mixes, Kits and Rtases List

31 Item(s)

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31 Item(s)

per page