Endotoxin Free Plasmid DNA Kits
Plasmid isolated with traditional protocol normally contains high level of endotoxins (Lipopolysaccharides or LPS). For transfection of endotoxin sensitive cell lines or microinjection, the endotoxins should be removed before the applications. BioVision offers ToxOutTM Endofree Plasmid Kits that uses a specially formulated buffer to efficiently remove endotoxin during or post plasmid DNA purification. Two rounds of extraction will reduce the endotoxin level to produce transfection grade plasmid DNA. The purified endotoxin free DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection.
Key Features:
- • Ready-To-Use Reagents
- • High Sensitivity: The endotoxin level after purification is <0.2 EU (Endotoxin) per µg of plasmid DNA
- • High Performance Downstream Applications: The purified endotoxin free DNA is ready for downstream applications such as transfection of endotoxin-sensitive cell lines, primary cultured cells or microinjection
Specifications
Typical Yield
ToxOut™ Endofree Plasmid Kits
Isolates endotoxin-free plasmid DNA for high efficiency transfection
ToxOut™ Endofree ezFilter Plasmid Kits
Isolates endotoxin-free plasmid DNA for high efficiency transfection in less than 60 minutes
