Tricarboxylic Acid Cycle-Kreb's Cycle-Citric Acid Cycle

The final common oxidative pathway for carbohydrates, fats and amino acids.

TCA cycle is the predominant source in all aerobic organisms to generate NADH and FADH2 from acetyl CoA, a product obtained by the decarboxylation of pyruvate. In addition, TCA cycle is also a major pathway for interconversion of metabolites and provides substrates for amino acid synthesis by transamination as well as for fatty acid synthesis and gluconeogenesis. The cycle starts with the condensation of acetyl-CoA with oxaloacetate to form citrate, a reaction catalyzed by citrate synthase. The entire cycle can be divided into two stages: a) decarboxylating stage involving conversion of citrate to succinyl-CoA and b) reductive stage involving successive oxidation of succinate to fumarate, fumarate to malate and malate to oxaloacetate.

Perturbation in the TCA cycle, whether caused by disease states, physical stress, or deficiencies in energy substrates leads to a wide range of metabolic disturbances and symptoms. Recent studies have even associated the dominant defects in three enzymes of TCA cycle namely, isocitrate dehydrogenase (IDH), succinate dehydrogenase (SDH) and fumarate hydratase (FH), with tumor formation. The molecular mechanisms linking the Kreb's cycle defects to tumor formation are now a major focus of research.

BioVision offers the assay kits for all the metabolites involved in the TCA cycle. These highly- sensitive and high-throughput adaptable assays are simple, rapid, and compatible with broad range of sample types.

Tricarboxylic Acid Cycle-Kreb's Cycle-Citric Acid Cycle Kits List