L-Amino Acid Assay Kit (ab65347)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 40 µM
Overview
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Product name
L-Amino Acid Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
<= 40 µM -
Assay time
0h 30m -
Product overview
L-Amino Acid Assay Kit ab65347 is used measure the level of L-amino acids in biological samples. There is no requirement for sample pretreatment or purification.
The L-amino acid(s) level can be quantified using fluorometric (at Ex/Em = 535/587 nm) or colorimetric (at λ = 570 nm) methods in 96-well plates.
Amino acid assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with microplate reader -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K639 L-Amino Acid Quantitation Colorimetric/Fluorometric Kit. K639-100 is the same size as the 100 test size of ab65347.
L-Amino acids are the most essential elements in biology. Accurately quantitating L-amino acids in body fluids or purified samples may provide valuable information for diagnostic or basic research studies.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer II 1 x 25ml L-Amino Acid Enzyme Mix 1 vial L-Amino Acid Standard 1 x 300µl OxiRed Probe 1 x 0.2ml -
Research areas
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Relevance
Amino acids perform various important functions for the body and serve as the materials for the body's cells, hormones, and enzymes. Of the standard amino acids, all but glycine can exist in either of the two optical isomers, called D and L enantiomers, which are mirror images of each other. The L-amino acids represent the vast majority of amino acids found in proteins during translation in the ribosome, and are therefore the most essential element in biology and metabolic functions.
Images
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab65347 has been referenced in 22 publications.
- Elmelund E et al. Opposing effects of chronic glucagon receptor agonism and antagonism on amino acids, hepatic gene expression, and alpha cells. iScience 25:105296 (2022). PubMed: 36325048
- Hope DCD et al. Hypoaminoacidemia underpins glucagon-mediated energy expenditure and weight loss. Cell Rep Med 3:100810 (2022). PubMed: 36384093
- Guimarães M et al. Can Metabolite and Hormone Profiles Provide a Rationale for Choosing Between Bariatric Procedures? Obes Surg 31:2174-2179 (2021). PubMed: 33511559
- Olennikov DN et al. Water-Soluble Melanoidin Pigment as a New Antioxidant Component of Fermented Willowherb Leaves (Epilobium angustifolium). Antioxidants (Basel) 10:N/A (2021). PubMed: 34439548
- Modvig IM et al. Amino acids differ in their capacity to stimulate GLP-1 release from the perfused rat small intestine and stimulate secretion by different sensing mechanisms. Am J Physiol Endocrinol Metab 320:E874-E885 (2021). PubMed: 33645250