Lyophilized Exosome Standard (Human Plasma) (ab288118)
Overview
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Product name
Lyophilized Exosome Standard (Human Plasma) -
Tested applications
Suitable for: Biochemical assaymore details -
General notes
This Lyophilized Exosome Standard (100 µg, Human Plasma) ab288118 was previously known as ExoStd Lyophilized Exosome Standard (100 µg, Human Plasma) M1041.
These lyophilized exosome standards are standardized positive controls for immunocapture performance evaluation. Lyophilization is the ideal technique for preserving the long-term stability of exosomes at 4 ºC. Lyophilized exosomes can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples. Lyophilized exosomes are easy to ship and store, and are stable for over 36 months at 4 °C. Purified and lyophilized exosomes are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva. Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis) with NanoSight LM10. Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids). These lyophilized exosome standards are highly pure, easy to reconstitute and easy to ship and store at 4ºC.
Properties
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Form
Lyophilized:Reconstitute the lyophilized exosome standard by adding deionized water. 100 µl for lyophilized standard 100 µg, to get a final concentration of 1 µg/µl. Suspend exosomes pipetting the solution up and down 10-15 times, avoiding bubbles. Vortex the reconstituted standard for 60 secs. Briefly centrifuge the tubes containing the standard to ensure that the solution is collected at the bottom of the tube. Pipette the solution up and down 10 times, avoiding bubbles. The standard is ready -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Prior to lyophilization, they are resuspended in PBS 1x, a small percentage of sugar (< 5%) is added as preservative for the lyophilization -
Concentration information loading...
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Biochemical assay |
Use at an assay dependent concentration.
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Notes |
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Biochemical assay
Use at an assay dependent concentration. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab288118 has not yet been referenced specifically in any publications.