Overall Exosome Capture and Quantification Assay Kit (ab287851)
Key features and details
- Assay type: Sandwich
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Cell culture supernatant
Overview
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Product name
Overall Exosome Capture and Quantification Assay Kit
See all CD9 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 13% -
Sample type
Cell culture supernatant -
Assay type
Sandwich -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Overall Exosome Capture and Quantification Assay Kit ab287851 (Previously known as ExoQuant Overall Exosome Capture and Quantification AssayKit (Cell Media, Colorimetric) Kit), is a double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes. It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from cell culture supernatants. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively)
Applications: Overall exosome immunocapture and quantification from cell culture supernatant
Features & Benefits: Double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 96 tests 1X Sample buffer 2 x 10ml 25X Washing buffer 1 x 25ml Exosome Standards 2 x 100µg HRP-Conjugate 1 x 5µl Immunoplate (Transparent) 1 unit Primary Antibody 1 x 20µl Stop Solution 1 x 10ml Substrate Chromogenic Solutions 1 x 10ml -
Research areas
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Function
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
Tissue specificity
Expressed by a variety of hematopoietic and epithelial cells. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Post-translational
modificationsProtein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Tetraspanin 29
- 5H9
- 5H9 antigen
see all -
Database links
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
Images
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CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.
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Example of standard curves obtained. Exosome standard preparation derived from COLO1 Cell Culture Supernatant
This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.
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Example of standard curves obtained. Exosome standard preparation derived from Plasma of healthy donors.
This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.
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CD9 titration of plasma healthy donor exosome standards.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab287851 has not yet been referenced specifically in any publications.