CRISPR-Cas9 Products

The bacterial adaptive immune system CRISPR (The Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR associated protein) has been used for the sequence-specific editing of mammalian genomes. The CRISPR system uses a Cas9 nuclease protein that complexes with a single guide RNA (sgRNA) containing a 20-nucleotide (nt) sequence for introducing site specific double-stranded breaks. Cells repair the break through one of two approaches, non-homologous end joining (NHEJ) or by homology-directed repair (HDR). Targeting the Cas9-sgRNA complex to DNA is specified by base pairing between the sgRNA and DNA as well as the presence of an adjacent NGG PAM (protospacer adjacent motif) sequence. Thus, CRISPR-Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows specific genomic disruption and replacement thereby providing an easy and quick way to modify the genome.

BioVision offers a wide range of CRISPR tools including the highly pure recombinant S. pyogenes and S. aureus Cas9 Proteins, antibodies, CRISPR Genomic Cleavage Detection Kit, and sgRNA Synthesis Kits for Genome Editing. Additionally, Biovision offers small molecule compounds that can effectively enhance or decrease CRISPR-mediated homology-directed repair (HDR) efficiency or can reduce off-target CRISPR-mediated gene editing.

Applications:

• RNA-guided Genome Editing
• Gene Regulation
• CRISPR Genomic Cleavage Detection Kit to Verify the Genomic Editing Process
• Simple CRISPR sgRNA Synthesis Kit for the Production of sgRNA

Key Features:

• Ready-To-Use, Highly Pure and Efficient S. pyogenes and S. aureus Cas9 Proteins
• Wide Range of Cas9 Proteins (Cas9 Nucleases, Nickases, Double Mutants) to Choose From
• Injection and Transfection Ready Cas9 Proteins
• GFP-Cas9 Proteins for Genomic Locus Visualization
• Ready-to-Use, Easy & Effective CRISPR Genomic Cleavage Detection Kit to Verify Genomic Editing
• Simple and Robust Production of sgRNA using CRISPR sgRNA Synthesis Kits
• Highly Specific Cas9 Monoclonal Antibody
• Small Molecule HDR Enhancers for enhancing CRISPR-mediated HDR efficiency
• Small Molecule HDR Blockers for decreasing CRISPR-mediated HDR efficiency