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ChIP validated Antibodies

BioVision is excited to offer a wide range of ChIP validated Antibodies

What is ChIP and how does it work?
Chromatin immunoprecipitation (ChIP) is a powerful and versatile research tool that is used to study how proteins interact with DNA in cells. Protein-DNA interactions have important implications in numerous mechanisms such as transcription of the gene, replication and recombination of DNA, segregation of chromosomes, silencing of the epigenome and signal transduction events. In addition to studying protein-DNA interactions, ChIP also helps to measure the relative abundance of a protein across the entire genome in vivo. During a ChIP assay, the cells are initially fixed with formaldehyde, which serves to cross-link and stabilize the protein-DNA interaction. This is followed by lysis of the cells, which is done either by enzymatic digestion or mechanical shearing such as sonication, to release smaller fragments of protein-DNA. These protein-DNA fragments are then immunoprecipitated with antibodies specific for the protein of interest. After immunoprecipitation, the cross-links of protein-DNA are reversed either by heat incubation or proteinase K digestion, the DNA is purified and analyzed by Polymerase Chain Reaction.

To successfully conduct ChIP experiments, antibodies used must be highly efficient, highly specific and of high quality. BioVision offers a wide range of antibodies that are validated for ChIP experiments.

 

Features & Benefits

Extensively validated in ChIP experiments
Highly specific
High quality antibodies
Available in Polyclonal and Monoclonal formats
Bulk Quotes available

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