Azide-free Antibodies
BioVision offers a large portfolio of Azide-free Antibodies. Antibody conjugation involves covalent binding of radioactive, fluorescent or enzyme labels to either monoclonal or polyclonal antibodies. Antibody conjugation is routinely performed to detect antigens in various research techniques. The most commonly used conjugates include horseradish peroxidase (HRP), alkaline phosphatase (AP), fluorescein isothiocyanate (FITC) and phycoerythrin (PE). Antibodies prepared in PBS, are preserved in sodium azide to prevent fungal or bacterial growth. However, antibodies preserved in sodium azide cannot be used for in vivo studies or stain live cells. Additionally, sodium azide cannot be used with HRP-conjugated antibodies. This is because azide reacts with HRP to form an azidyl radical thereby irreversibly inhibiting the HRP enzyme activity. BioVision offers a wide range of Azide-free Antibodies for antibody conjugation, live cell assays and in vivo studies.
