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Procollagen Ⅰ N-Terminal Propeptide (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of PINP in human serum, plasma and other biological fluids

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to
Catalog #: E4756

Product Details

Cat # +Size E4756-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Quantitative measurement of PINP in human serum, plasma and other biological fluids
Features & Benefits • Sensitivity: 9.38 pg/mL
• Quantitative measurement of PINP in human serum, plasma and other biological fluids
• Precision: Coefficient of variation is < 10%.
• Detection Range: 15.63-1000 pg/mL
Kit Components HRP Conjugate Diluent
Plate Sealer
Stop Solution
Wash Buffer (25x)
HRP Conjugate (100x)
Biotinylated Detection Antibody Diluent
Micro ELISA Plate
Standard and Sample Diluent
Biotinylated Detection Antibody (100x)
Substrate Reagent
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s Procollagen I N-Teminal Propeptide (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PINP. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human PINP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of PINP bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PINP. The concentration of Human PINP in the samples can be calculated by comparing the OD of the samples to the standard curve.

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