Product Details
| abID | ab285906 |
|---|---|
| Alternate Name | CTSL, CTSL1 |
| Gene Symbol | CTSL1 |
| Gene ID | 1514 |
| Accession # | P07711 |
| Source | E. coli |
| Appearance | Liquid |
| Physical Form Description | 1 mg/ml in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0. |
| Molecular Weight | 39.4 kDa (18-333 aa + N-terminal poly-his tag) |
| Purity by SDS-PAGE | ≥90% |
| Biological Activity | > 10000 mU/mg (1 U = digestion of 1 µmole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37°C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5). |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry Ice |
| USAGE | For Research Use Only! Not to be used in humans |
Details
Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. BioVision’s Procathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
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