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Procathepsin L, Human Recombinant

A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
Catalog #: 7581
SKU-Size Size Price Qty
7581-5 5 μg
$195.00
7581-100 100 μg
$995.00
7581-1000 1 mg
$4,450.00
More Sizes Get Quote

Product Details

Alternate Name CTSL, CTSL1
Gene Symbol CTSL1
Gene ID 1514
Accession # P07711
Source E. coli
Appearance Liquid
Physical Form Description 1 mg/ml in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.
Molecular Weight 39.4 kDa (18-333 aa + N-terminal poly-his tag)
Purity by SDS-PAGE ≥90%
Biological Activity > 10000 mU/mg (1 U = digestion of 1 µmole of Z-Phe-Arg-AFC substrate (K142-100) per minute at 37°C in 50 mM Sodium Acetate, 100 mM NaCl, pH 5.5).
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. BioVision’s Procathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.


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