Procathepsin B, Human Recombinant

A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
Catalog #: 7579 | abID:

Product Details

Alternate Name CTSB, CPSB
Gene Symbol CTSB
Gene ID 1508
Accession # P07858
Source E. coli
Appearance Lyophilized
Physical Form Description Lyophilized from a proprietary buffer.
Molecular Weight 38.2 kDa (18-339 aa + N-terminal poly-his tag)
Purity by SDS-PAGE ≥95%
Biological Activity > 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2.
Reconstitution Instructions Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans


Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

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