Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Procathepsin B, Human Recombinant

A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.
Catalog #: 7579
SKU-Size Size Price Qty
7579-10 10 μg
$239.00
7579-50 50 μg
$895.00
7579-1000 1 mg
$7,950.00
More Sizes Get Quote

Product Details

Alternate Name CTSB, CPSB
Gene Symbol CTSB
Gene ID 1508
Accession # P07858
Source E. coli
Appearance Lyophilized
Physical Form Description Lyophilized from a proprietary buffer.
Molecular Weight 38.2 kDa (18-339 aa + N-terminal poly-his tag)
Purity by SDS-PAGE ≥95%
Biological Activity > 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2.
Reconstitution Instructions Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles.
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.