Product Details
| Alternate Name | CTSB, CPSB |
|---|---|
| Gene Symbol | CTSB |
| Gene ID | 1508 |
| Accession # | P07858 |
| Source | E. coli |
| Appearance | Lyophilized |
| Physical Form Description | Lyophilized from a proprietary buffer. |
| Molecular Weight | 38.2 kDa (18-339 aa + N-terminal poly-his tag) |
| Purity by SDS-PAGE | ≥95% |
| Biological Activity | > 5000 mU/mg (1 U = 1 µmole/min, after complete activation) of Ac-Arg-Arg-AFC substrate (K140-100) at 37°C in 50 mM Tris, 1 mM EDTA, 10 mM DTT, pH 7.2. |
| Reconstitution Instructions | Reconstitute in de-ionized water to 1 mg/ml and store at –20°C. Avoid repeated freezing and thawing cycles. |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer. Biovision’s Procathepsin B can be auto-catalytically activated to mature Cathepsin B (~28 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at room temperature (RT). The enzyme is fully activated within 3-4 h of incubation at RT. After activation mature Cathepsin B is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1 mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.
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