Recombinant Human Procalcitonin protein (ab92843)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Suitable for: HPLC, SDS-PAGE, WB
Description
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Product name
Recombinant Human Procalcitonin protein
See all Procalcitonin proteins and peptides -
Purity
> 95 % SDS-PAGE.
>95% as determined by RP-HPLC analyses. Purified using standard chromatographical techniques. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN -
Predicted molecular weight
13 kDa -
Amino acids
26 to 141 -
Additional sequence information
Mature protein without signal peptide.
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Specifications
Our Abpromise guarantee covers the use of ab92843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
HPLC
SDS-PAGE
Western blot
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituent: 0.16% Sodium phosphate
Lyophilized from a sterile (0.2 micron) filtered aqueous solution. -
ReconstitutionReconstitute at 0.1 mg/ml with sterile water. Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
General Info
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Alternative names
- CALC1
- CALCA
- calcitonin related polypeptide alpha
see all -
Relevance
Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis. -
Cellular localization
Secreted
Images
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1ug of ab92843 was resolved on a 4-20% polyacrylamide, Tris-Glycine gel under non-reducing and reducing (+) conditions Recombinant Human Procalcitonin has a predicted molecular weight of 12.8kDa.
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ab92843 used in Western Blot. Figure: 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue.
Human Procalcitonin has a predicted MW of 12.8 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab92843 has been referenced in 1 publication.
- Alshawawreh F et al. The use of a personal glucose meter for detecting procalcitonin through glucose encapsulated within liposomes. Analyst 144:6225-6230 (2019). PubMed: 31555776