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Presepsin (Human) ELISA Kit

A Sandwich ELISA kit for the qualitative detection of Presepsin in human serum or plasma.
Catalog #: E4696

In stock

$695.00

Product Details

Cat # +Size E4696-100
Size 96 assays
Detection Method Colormetric
Species Reactivity Human
Applications • In vitro quantitative determination of Presepsin in human samples.
Features & Benefits • Easy, convenient and time-saving method to measure the level of Presepsin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
• No significant cross-reactivity or interference was observed,Sensitivity: < 0.094 ng/ml
• Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
• Detection Range: 0.156-10 ng/ml
Kit Components • Biotin- detection antibody
• Antibody dilution buffer
• Sample / Standard dilution buffer
• Plate sealers
• Lyophilized Standard
• TMB substhumane (Avoid light)
• HRP-Streptavidin Conjugate (SABC) (Avoid light)
• Wash buffer (25X)
• Micro ELISA Plate
• SABC dilution buffer
• Stop Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Presepsin is a soluble subtype CD14. It is considered a novel biomarker for sepsis diagnostic and prognosis evaluation. BioVision’s Presepsin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Presepsin in human serum, plasma and cell culture supernatants. Anti-Presepsinantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Presepsin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Presepsin amount of sample captured in plate.


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