Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Preadipocyte Isolation Kit

Only kit on the market to isolate preadipocytes from adipose tissue
Catalog #: K583

In stock


Product Details

Cat # +Size K583-5
Size For 5 g Tissue
Applications Isolation of preadipocytes from adipose tissue
Features & Benefits • Simple, rapid & convenient method to isolate preadipocytes from adipose tissue.
Kit Components • Collagenase (0.2%)
• Collagenase Stop Buffer
• Adipocyte Wash Buffer
• Adipocyte Lipolysis Buffer
• Glycerol Assay Buffer
• Glycerol Probe (in DMSO, anhydrous)
• Glycerol Enzyme Mix (Lyophilized)
• Glycerol Standard (100 mM)
• Isoproterenol (10 mM)
• Cell strainer (100 µm)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Adipose tissue is loose connective tissue that accumulates in animals and serves primarily for energy storage, insulation, and thermoregulation. It is predominantly composed of adipocytes, but also contains a stromal vascular fraction consisting of preadipocytes, fibroblasts, vascular endothelial cells, and immune cells. Recent research has discovered a dynamic role of adipose tissue in the production and secretion of various hormones and as an active endocrine organ. The excessive accumulation of adipose is known as obesity. Ways to prevent over-accumulation of adipose is a field of intense research. In addition to obesity, adipose tissue has been found to participate in various physiological processes, including reproduction, angiogenesis, inflammation, cancer, and vascular homeostasis. BioVision’s Preadipocyte Isolation Kit provides the reagents and tools to isolate the stromal vascular fraction from adipose tissue. The resulting stromal vascular fraction is cultured on a tissue-culture plate, where preadipocytes adhere. The preadipocytes retain the ability to proliferate and differentiate into adipocytes when treated with differentiation-inducing components (Cat. # K579, 3T3-L1 Differentiation Kit). The preadipocytes can be used to study the process of adipogenesis and the differentiated adipocytes can be used to study lipolysis, endocrine activity, cell-signaling, and metabolic dysfunction.

Why buy BioVision Products?
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.