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PPARG (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of PPAR gamma in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E4992
$750.00

Product Details

Cat # +Size E4992-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure PPARG in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits ● This Sandwich ELISA is highly sensitive and highly specific for the detection of PPARG in human samples. There is no significant cross-reactivity or interference between PPARG and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Detection range: 0.156 – 10 ng/ml
● Sensitivity: 0.094 ng/ml
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (10 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Peroxisome proliferator-activated receptor-γ (PPARG) is a type II nuclear hormone receptor that belongs to the family of Peroxisome proliferator-activated receptors. This ligand-activated transcription factor is mainly present in the liver, macrophages, adipose tissue, and colon. PPARG exists in 2 isoforms in humans and mice: PPARG1 (all tissues except muscle) and PPARG2 (in adipose tissue and intestine). PPARG forms heterodimers with retinoid X receptor (RXR), binds to the consensus DR1 sequence in the promoters of target genes, and regulates their transcriptional activity. PPARG is known to promote differentiation and survival of adipocytes, lipogenesis, and regulate insulin sensitivity. PPARG also plays a critical role in the circadian rhythms. Circadian expression of PPARG has been reported in mouse liver, blood vessels, and adipose tissue. PPARG also regulates circadian clock components such as Bmal1 and Rorg. Deletion of PPARG dampens or abrogates these rhythms and disturbs metabolic and energy homeostasis. PPARG is implicated in the pathogenesis of several diseases such as diabetes, atherosclerosis, obesity, and cancer. Therefore PPARG could serve as a promising target to treat metabolic diseases. BioVision’s PPARG (Human) ELISA kit quantitatively measures PPARG in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of PPARG in the sample or standard.


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