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PPARγ (LBD) Human Recombinant (His-tagged)

Recombinant Human PPARγ (LBD) can be used in the ligand screening assays, selectivity profiling, western blotting, ELISA, and numerous such applications
Catalog #: 7878
SKU-Size Size Price Qty
7878-20 20 μg
$105.00
7878-100 100 μg
$385.00
7878-500 500 μg
$1,325.00
More Sizes Get Quote

Product Details

Alternate Name PPAR-gamma, Peroxisome Proliferator-activated Receptor γ Ligand Binding Domain, PPAR-gamma (LBD), NR1C3, PPARG (LBD)
Gene Symbol PPARG
Gene ID 5468
Source E. coli
Appearance Liquid. In 50 mM Tris, 100 mM NaCl, 1 mM TCEP, pH 8.0 containing 20% glycerol
Molecular Weight 34.8 kDa (NP_619726.2, 193–477 aa + N-terminal His6- tag)
Purity by SDS-PAGE ≥90%
Reconstitution Instructions It can be further diluted to 0.5 mg/ml with 50 mM Tris, 100 mM NaCl, 1 mM TCEP, pH 8.0 containing 20% glycerol and stored at –80°C in aliquots. Avoid repeated freezing and thawing cycles.
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

The peroxisome proliferator activated receptor (PPAR) family of ligand-activated transcription factors consists of three subtypes encoded by separate genes: PPARα, PPARδ and PPARγ. Of these, PPARγ plays an important role in the regulation of fatty acid storage and glucose metabolism. The genes activated by PPARγ stimulate lipid uptake and adipogenesis by fat cells. Many naturally occurring molecules such as, polyunsaturated fatty acids like arachidonic acid and arachidonic acid metabolites, are known to bind and activate PPARγ. The binding of activating ligands to the ligand binding domain (LBD) of PPARγ promotes its heterodimerization with retinoic acid-like receptor (RXR), which results in the regulated expression of target genes involved in lipid metabolism. BioVision’s His-PPARγ (LBD) is a non-glycosylated protein expressed and purified from E. coli. It has been tested for functionality by its binding and subsequent displacement of 1,8-ANS assay.


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