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PNGase F Deglycosylation Kit

For the quick deglycosylation of glycoproteins using PNGase F under optimized conditions
Catalog #: K2061
$415.00

Product Details

Cat # +Size K2061-100
Size 100 assays
Kit Summary BioVision’s PNGase F Deglycosylation Kit is designed for the quick deglycosylation of glycoproteins using PNGase F under optimized conditions. The kit provides all the necessary reagents for the deglycosylation at least 20 mg of glycoproteins.
Detection Method SDS-PAGE Analysis
Species Reactivity All
Applications • Structural and functional study of N-linked glycoproteins.
• Preparation of glycans and proteins of glycoprotein for mass analysis, crystallization and structural studies.
• Removal of glycans from N-linked glycopeptides and glycoproteins.
Features & Benefits • The kit provides all the necessary reagents for the deglycosylation at least 20 mg of glycoproteins.
• For the quick deglycosylation of glycoproteins using PNGase F under optimized conditions.
Kit Components • PNGase F Reaction Buffer (5X)
• Denaturation Buffer (10X)
• Detergent Solution (10X)
• Recombinant PNGase F
• Glycoprotein Control
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Peptide-N4-(N-acetyl-β-D-glucosiminyl asparagine amidase F (PNGase F) is an amidohydrolase that cleaves the N-glycan chains at the innermost N-Acetylglucosamine (GlcNAc) of glycans and asparagine residues of high mannose, hybrid and complex oligosaccharides of N-linked glycoproteins and glycopeptides. The cleavage results in a deaminated protein or peptide and free glycans. PNGase F is widely used in the deglycosylation of glycoproteins to study glycans and identify glycosylation sites. BioVision’s PNGase F Deglycosylation Kit is designed for the quick deglycosylation of glycoproteins using PNGase F under optimized conditions. The kit provides all the necessary reagents for the deglycosylation at least 20 mg of glycoproteins.


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