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PM20D1 Activity Assay Kit (Colorimetric)

For detecting the activity of PM20D1 in various sample types
Catalog #: K2107
$545.00

Product Details

Cat # +Size K2107-100
Size 100 assays
Kit Summary BioVision’s PM20D1 Activity Assay Kit is a simple, rapid and sensitive assay to detect the activity of PM20D1 in various sample types.
Detection Method Absorbance at 570 nm
Applications ● Mechanistic studies of various human diseases. i.e. Obesity, Alzheimer’s Disease etc.
● Measurement of PM20D1 activity in various sample types
Features & Benefits ● A simple, rapid and sensitive assay to detect the activity of PM20D1 in various sample types.
● The assay can detect less than 0.1 mU under the assay conditions.
Kit Components ● PM20D1 Assay Buffer
● PM20D1 Probe
● PM20D1 Substrate
● PM20D1 Converter
● PM20D1 Developer
● PM20D1 Positive Control
● Glycine Standard (0.88 M)
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Peptidase M20 domain containing 1 (PM20D1), also known as N-fatty-acyl-amino acid synthase/hydrolase, is a secreted enzyme that catalyzes the biosynthesis of N-fatty acyl amino acids (NAAs) from free amino acids and free fatty acids and also catalyzes the reverse hydrolysis reaction. NAAs play an important role in various physiological functions as lipid signaling molecules. Additionally, they can stimulate oxidative metabolism via mitochondrial uncoupling. Mouse and human PM20D1 share about 71% amino acid identity. PM20D1 is involved in energy homeostasis. Ablation of PM20D1 in mice has shown bidirectional dysregulation of circulating NAAs, insulin resistance, altered body temperature, glucose intolerance etc. PM20D1 has also been associated with Alzheimer’s disease. Therefore, studying PM20D1 activity is of great interest in treating obesity, obesity-related disorders and neurological diseases. BioVision’s PM20D1 Activity Assay Kit is a simple, rapid and sensitive assay to detect the activity of PM20D1 in various sample types. In this assay, PM20D1 catalyzes the conversion of N-Oleoyl-Glycine to generate glycine. In the subsequent steps, glycine in the presence of converter and developer generate hydrogen peroxide, which reacts with the PM20D1 probe to generate color measured at OD 570 nm. The assay can detect less than 0.1 mU under the assay conditions.


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