You have no items in your shopping cart.
Product Details
| Cat # +Size | K593-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em 465/535 nm) |
| Applications | Measurement of PLTP activity in animal serum, plasma and recombinant protein |
| Features & Benefits | • Rapid and convenient • Includes Positive Control (rabbit serum) |
| Kit Components | • PLTP Assay Buffer • Donor Molecule (30 nmol/ml) • Acceptor Molecule • Positive Control (rabbit serum) |
| Storage Conditions | 4°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Phospholipid transfer protein (PLTP) plays an important role in transferring phospholipids between HDL molecules to modulate HDL size and composition and controlling plasma HDL levels. PLTP also transfers phospholipids from lipoproteins to HDL. PLTP plays a key role in reverse cholesterol transport and may promote atherosclerosis. PLTP is, therefore, considered a promising target for pharmacological intervention. BioVision’s PLTP Activity Fluorometric Assay Kit II uses a self-quenched fluorescent phospholipid that can be measured when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of phospholipid transferred. Rabbit serum is provided as a positive control. This Assay Kit, in addition to measuring activity in serum, is also suitable for testing activity of recombinant protein.
Why buy BioVision Products?
 |
 |
 |
 |
Innovation |
Affordability |
Global Presence |
Technical Support |
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
BioVision is committed to providing the highest quality products at a competitive price. |
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery. |
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently. |
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can we use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact yopur regional BioVision distributor.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are my standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, you can increase the incubation time. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How do I normalize my samples against protein concentration
You can use a protein quantitation assay on the supernatants you get from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Should I make a standard curve for every expt I do, or is one curve/kit enough?
Yes, I would strongly recommend you to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Should we use human serum or human plasma with the kit?
Although, Serum and plasma PLTP values are not supposed to be very different, plasma is recommended.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
