Plasminogen Activator Inhibitor-1 Activity Assay Kit (C)

To measure PAI-1 activity in Biological Samples

K2040 is available from Abcam as ab283368.
Catalog #: K2040 | abID: ab283368

Product Details

abID ab283368
Cat # +Size K2040-100
Size 100 assays
Detection Method Absorbance, OD 450 nm
Species Reactivity All
Applications • Analysis and study of fibrinolytic system
• Measurement of PAI-1 activity in Biological Samples such as plasma, cell culture medium
Features & Benefits • Specific
• Simple
• Can detect as low as 2 U/ml of PAI-1 activity in samples
Kit Components • PAI-1 Assay Buffer
• PAI-1 Acidify Buffer
• PAI-1 Stop Buffer
• Human tPA
• Plasminogen
• PAI-1 Substrate Mix
• Clear 96-well half area plate
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Plasminogen Activator Inhibitor-1 (PAI-1), also known as endothelial plasminogen activator inhibitor is a serine protease inhibitor that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). PAI-1 is present in a number of tissues & cell types and is associated with various thrombotic and fibronolytic complications. High levels of PAI-1 activities are found in patients suffering from myocardial infarction, hemolytic uremic syndrome or stroke whereas low levels of PAI-1 activity are associated with bleeding disorders. Additionally, PAI-1 is a prognosticator in various cancers. BioVision’s Plasminogen Activator Inhibitor-1 Activity Assay Kit is a two-step colorimetric assay. In the first step, samples are incubated with a known amount of tPA thereby allowing PAI-1 and tPA to form an inactive complex. In the second step, the residual tPA converts plasminogen to plasmin, which in turn hydrolyzes the plasmin chromogenic substrate thereby releasing pNA (chromophore). The absorbance of the released pNA is inversely proportional to the PAI-1 activity in the samples. The assay kit is simple, specific and can detect as low as 2 U/ml of PAI-1 activity in samples.

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