Product Details
| abID | ab283368 |
|---|---|
| Cat # +Size | K2040-100 |
| Size | 100 assays |
| Detection Method | Absorbance, OD 450 nm |
| Species Reactivity | All |
| Applications | • Analysis and study of fibrinolytic system
• Measurement of PAI-1 activity in Biological Samples such as plasma, cell culture medium |
| Features & Benefits | • Specific
• Simple • Can detect as low as 2 U/ml of PAI-1 activity in samples |
| Kit Components | • PAI-1 Assay Buffer
• PAI-1 Acidify Buffer • PAI-1 Stop Buffer • Human tPA • Plasminogen • PAI-1 Substrate Mix • Clear 96-well half area plate |
| Storage Conditions | -20ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Plasminogen Activator Inhibitor-1 (PAI-1), also known as endothelial plasminogen activator inhibitor is a serine protease inhibitor that functions as the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA). PAI-1 is present in a number of tissues & cell types and is associated with various thrombotic and fibronolytic complications. High levels of PAI-1 activities are found in patients suffering from myocardial infarction, hemolytic uremic syndrome or stroke whereas low levels of PAI-1 activity are associated with bleeding disorders. Additionally, PAI-1 is a prognosticator in various cancers. BioVision’s Plasminogen Activator Inhibitor-1 Activity Assay Kit is a two-step colorimetric assay. In the first step, samples are incubated with a known amount of tPA thereby allowing PAI-1 and tPA to form an inactive complex. In the second step, the residual tPA converts plasminogen to plasmin, which in turn hydrolyzes the plasmin chromogenic substrate thereby releasing pNA (chromophore). The absorbance of the released pNA is inversely proportional to the PAI-1 activity in the samples. The assay kit is simple, specific and can detect as low as 2 U/ml of PAI-1 activity in samples.
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