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Plasmin Inhibitor Screening Kit (Fluorometric)

Validated using aprotinin inhibitor
Catalog #: K382
$465.00

Product Details

Cat # +Size K382-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 360/450 nm)
Applications Screening potential inhibitors of plasmin
Characterize/study plasmin inhibitors
Features & Benefits • Rapid, simple & reliable test for screening potential inhibitors of plasmin
• Includes plasmin Inhibitor [Aprotinin]
Kit Components • Plasmin Assay Buffer
• Plasmin Dilution Buffer
• Plasmin Enzyme
• Plasmin Substrate
• Plasmin Inhibitor (Aprotinin, 0.6 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasmin inhibitors are critical in the treatment of hyperfibrinolysis-associated blood loss and related complications. Biovision’s Plasmin Inhibitor Screening Kit utilizes the ability of Plasmin to cleave a synthetic AMC-based peptide substrate to release AMC, which can be detected by measuring its fluorescence at Ex/Em = 360/450 nm. In the presence of Plasmin specific inhibitors, the extent of cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The kit provides a simple and rapid method to screen potential inhibitors of Plasmin.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
May I confirm if the plasmin enzyme supplied in K382-100 is already in active form, so this Activation Buffer mentioned in Figure b is typically not required? In other words, this buffer is only required if native plasma (crude) is used for the assay.
Yes, the kit is supplied with Plasmin enzyme that is in its active form and you do not need to activate any component, unless, it is mentioned in the data sheet.
May I know if Catalog #: 7696 (Urokinase, human recombinant) will be able to cleave plasminogen to form active plasmin of different species of origin (say, human, mouse, rat. hamster)?
Yes, Urokinase is an active enzyme and activity was estimated based on its ability to cleave a fluorogenic substrate N-carbobenzyloxy-Gly-Gly-Arg-7- amido-4-methylcoumarin (Z-GGR-AMC). We used Cat # K728-100 to determine the activity of Urokinase.