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Plant Tissue Genomic DNA Isolation Kit

Isolates highly pure, intact plant DNA

WARNING: This product can expose you to chemicals including Silica, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: K316
$300.00

Product Details

Cat # +Size K316-100
Size 100 isolations
Detection Method 0
Species Reactivity Plants
Applications Useful for PCR, Cloning, DNA hybridization, Southern Blotting
Features & Benefits • Simple & rapid method to isolate highly pure, intact DNA from plant tissues
Kit Components • Buffer L [Lysis Buffer]
• 1 M DTT
• Enzyme Mix
• RNAse A
• Buffer B [Binding Buffer]
• Buffer W [Wash Buffer]
• Buffer E [Elution Buffer]
• Spin Columns/Collection Tubes
Storage Conditions -20°C/RT
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Plants serve a vital role in human nutrition, biofuels, and the ecosystem. Isolating DNA from plants is exceptionally challenging compared to other organisms due to the presence of difficult to disrupt cell wall and compounds such as tannins, phenolics, and complex polysaccharides that can affect DNA quality and inhibit downstream reactions. BioVision’s Plant Genomic DNA Isolation kit enables the researchers to isolate genomic DNA from plant leaves, stalks, seeds, and roots. This kit utilizes an efficient combination of lysis buffer and enzymes that work in unison to disrupt the cell wall, eliminate plant specific contaminating compounds, and release free genomic DNA. DNA release from the cell is coupled with adsorption of DNA onto a silica spin-column under chaotropic conditions, eliminating the use of toxic organic compounds or solvents. This kit yields pure DNA that is suitable for various downstream molecular biology applications such as PCR, cloning, sequencing, DNA hybridization and Southern Blotting, etc.


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What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.