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PicoProbe™ Tyrosine Aminotransferase Activity Assay Kit (Fluorometric)

Sensitive Assay, HTS
Catalog #: K486
$455.00

Product Details

Cat # +Size K486-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The kit detects TAT activity with a detection limit of 4 µU per well.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity Eukaryotes
Applications Measurement of TAT activity in cell and tissue lysates using a 96-well plate format
Features & Benefits • Simple procedure
• Fast and convenient
• Sensitive assay for measuring TAT activity in various biological samples, high-throughput compatible
Kit Components • TAT Assay Buffer
• TAT Substrate I
• TAT Substrate II
• TAT Developer
• TAT Enzyme Mix
• TAT Probe
• TAT Probe
• Glutamate Standard (0.1 M)
• TAT Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Tyrosine Aminotransferase (EC 2.6.1.5), also known as Tyrosine Transaminase (TAT), is a mitochondrial enzyme encoded by the TAT gene in the nuclear DNA. It catalyzes the transamination of tyrosine by transferring an amine group to an α-ketoglutarate molecule. Tyrosine Aminotransferase is highly expressed in the liver and its deficiency, usually inherited, leads to a buildup of toxic levels of tyrosine in the body called tyrosinemia. Tyrosinemia causes skin and corneal lesions, kidney and liver disorders, and could lead to mental retardation in newborns. BioVision’s PicoProbe™ Tyrosine Aminotransferase Activity Assay Kit is a simple plate-based fluorometric assay for the measurement of TAT activity in biological samples. The transamination of tyrosine by TAT produces glutamate (through the transfer of amine group to α-ketoglutarate), which converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. The assay can detect as low as 4 µU of tyrosine aminotransferase.


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