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PicoProbe™ Reduced Glutathione (GSH) Assay Kit (Fluorometric)

It specifically detects reduced glutathione. Oxidized GSSG is not detected.
Catalog #: K740
$475.00

Product Details

Cat # +Size K740-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (535/587 nm)
• Species reactivity- Mammalian
• Applications- The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
Detection Method Detection method- Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting reduced GSH levels in apoptosis and other pathological processes. Oxidized GSSG is not detected.
Features & Benefits • Simple procedure; takes only ~1-2 hours
• Fast and convenient
• Specific: does not detect oxidized GSSG
• Sensitive: detects as low as 8 pmol GSH
Kit Components • GSH Assay Buffer
• PicoProbeTM (in DMSO)
• Substrate Mix A
• Substrate Mix B
• Enzyme Mix A
• Enzyme Mix B
• Enzyme Mix C
• Sulfosalicylic Acid (SSA, 1 gram)
• GSH Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glutathione (GSH), a thiol-containing tripeptide, is a key antioxidant in living systems. Intracellular GSH status appears to be a sensitive indicator of the overall health of a cell. BioVision’s PicoProbe™ Reduced Glutathione Assay Kit is based on a specific enzymatic cycling method in the presence of GSH and a fluorophore. The reduction of the fluorophore produces a stable fluorescent product, the fluorescence of which is directly proportional to the amount of GSH in the sample and can be followed kinetically (Ex/Em=535/587 nm). Our assay is the most specific and sensitive in the market. Oxidized Glutathione does not interfere with the assay. The assay is simple, reproducible and can specifically detect as low as 8 pmol/well of reduced form of Glutathione (GSH) in a 100 µl reaction.


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