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New

PicoProbe™ Proline Assay Kit (Fluorometric)

for measuring proline concentrations
Catalog #: K2020
$495.00

Product Details

Cat # +Size K2020-100
Size 100 assays
Kit Summary BioVision’s PicoProbe™ Proline Assay Kit provides a quick, specific and easy method for measuring proline concentrations in a wide variety of samples. In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 5 pmol of proline per well.
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity All
Applications • Assaying proline concentration as a stress factor in plants.
• Analyzing maturation of honey.
• Measurement of proline in biological samples and honey.
Features & Benefits • Specific
• Easy method for measuring Proline concentrations in a wide variety of samples.
• Quick
Kit Components Proline Assay Buffer
PicoProbe™
Proline Enzyme Mix
Proline Cofactor Mix
Proline Developer Mix
Proline Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Proline is a proteogenic amino acid, which plays an important role in protein folding. In humans, proline can be synthesized from glutamate and arginine. Proline is one of the conditionally essential amino acids in humans. Proline is also produced by honey bees as they process nectar into honey. Thus, its content in honey is often used as an indicator of honey ripeness and sugar adulteration. For mature honey, 180 mg-proline/kg of honey is used as an international standard. Proline contents are measured for its biological importance in plants where it also serves as a stress marker. Unstressed plants contain approximately 0.5 µmol proline per gram of plant tissue while stressed plants can range up to 100 times more proline than the unstressed ones. BioVision’s PicoProbe™ Proline Assay Kit provides a quick, specific and easy method for measuring proline concentrations in a wide variety of samples. In this assay, proline is converted to an intermediate, which will further react with a probe to produce a strong fluorometric signal (Ex/Em = 535/587 nm). The kit is simple, easy to use, sensitive and high-throughput adaptable. It can detect as low as 5 pmol of proline per well.


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