Product Details
| abID | ab252900 |
|---|---|
| Cat # +Size | K750-100 |
| Size | 100 assays |
| Detection Method | Fluorometric (Ex/Em = 535/587 nm) |
| Species Reactivity | All |
| Applications | Measurement of PI content of various tissue/cell extracts,Determination of PI concentration in biological fluids |
| Features & Benefits | • This assay provides a convenient and non-ELISA alternative to measure phosphatidylinositol concentration in biological samples. • Validated (biological fluids, tissue/cell lysate) • Sensitive, Specific |
| Kit Components | • PI Standard (0.2 µmol) • PicoProbeTM (in DMSO) • PI Hydrolysis Mix • Sample Clean-Up Mix • PI Developer • PI Assay Buffer • PI Enzyme Mix |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Phosphoinositides are critical second messengers in a multitude of cellular processes. The most fundamental phosphoinositide, phosphatidylinositol (PI), can be phosphorylated at multiple sites around its inositol ring, leading to downstream activation. For instance, phosphatidylinositol 4-[mono]phosphate is involved in membrane trafficking, while cleavage of phosphatidylinositol triphosphate yields inositol triphosphate, a molecule involved in calcium signaling. Signaling is conveyed in many signaling proteins (including Protein Kinase B/Akt) via their domain referred to as a Pleckstrin homology, or PH domain, which is capable of binding to specific phosphoinositides. Biosynthesis of PI involves the action of phosphatidylinositol synthase on cytidine diacylglycerol and myo-inositol. The critical signal transducer enzymes, Phosphatidylinositolinositol-3-kinases phosphorylate PI at the 3 position hydroxyl of inositol, leading to modulation in pathways such as cell growth, survival, and motility. PI is typically at concentrations in the low- to mid-micromolar range in human serum and plasma, and is capable of activating second messengers such as Ca2+ and cyclic AMP. It is through these pathways that PI has been found to affect such biological events as the pro-inflammatory response and intestinal uptake. BioVision’s Phosphatidylinositol Assay Kit utilizes specific enzymes to generate an intermediate that then reacts with PicoProbe™, yielding a signal that can be quantified fluorometrically, and is proportional to the amount of PI present in the sample. The assay is capable of detecting as little as 15 pmoles of phosphatidylinositol.
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