PicoProbe™ Phosphate Fluorometric Assay Kit

Highly Sensitive Assay

K419 is available from Abcam as ab273281.
Catalog #: K419 | abID: ab273281

Product Details

abID ab273281
Cat # +Size K419-100
Size 100 assays
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity All
Applications PicoProbe assay is highly sensitive with the detecting limit approximately 40 pmol/well.
Features & Benefits • Simple procedure; takes ~ 3 hours
• Fast and convenient
• The assay is sensitive and stable
• The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Kit Components • Phosphate Assay Buffer
• PicoProbe™
• Converter Enzyme
• Developer Enzyme
• Substrate Mix
• Phosphate Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. The Pi ion is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to runoff from areas of high fertilizer use. The PicoProbe™ Phosphate Assay Kit provides an easy, quick and sensitive means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate will react with substrate and PicoProbe™ to generate fluorescence (Ex/Em = 535/587 nm). The kit can be used to detect Pi in a variety of samples or to monitor phosphate released by an assortment of enzymes (such as ATPases, GTPases, 5’-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase kinase). Unlike other commercially available assays, the PicoProbe™ assay is not affected by the presence of glucose in samples (Note: Glucose interferes with many other commercially available assays). This PicoProbe™ assay is highly sensitive with the detecting limit approximately 40 pmol/well.

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Can lipid phosphate be detected, e.g.sphingosine-1-phosphate ?
Inorganic phosphate ion (Pi) in solution is detected by this kit, not lipid phosphates. If the phosphate dissociates from the lipid in solution leading to Pi then it will be detected.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.