NADPH Fluorometric Kit (ab287848)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate (12 x 8 well strips)
- Sample type: Cell culture media, Purified mitochondria, Tissue Extracts
- Sensitivity: 40 nM
Overview
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Product name
NADPH Fluorometric Kit
See all NADPH kits -
Detection method
Fluorescent -
Sample type
Tissue Extracts, Cell culture media, Purified mitochondria -
Assay type
Quantitative -
Sensitivity
< 40 nM -
Assay duration
Multiple steps standard assay -
Product overview
NADP (nicotinamide dinucleotide) is a major electron donor for anabolic reactions such as lipid and nucleic acid synthesis. The reduced form, NADPH is a cofactor for many redox enzymes. It is of increasing interest to be able to measure low level of intracellular NADPH. The New NADPH Quantitation Assay kit ab287848 (Previously known as PicoProbe NADPH Quantitation Fluorometric Assay Kit) provides a highly sensitive method to detect intracellular NADPH. NADH is not detected as the NADPH Recycling Enzyme Mix specifically recognizes NADPH in the enzyme recycling reaction. The assay is rapid, simple and does not require purifying NADPH from samples. The kit can measure less than 40 nM NADPH in a variety of samples.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K349 PicoProbe™ NADPH Quantitation Fluorometric Assay Kit. K349-100 is the same size as the 100 test size of ab287848.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests NADPH Cycling buffer 1 x 15ml NADPH Cycling Enzyme Mix 1 x 0.2ml NADPH Extraction buffer 1 x 50ml NADPH Standard (Lyophilized) 1 vial PicoProbe 1 x 0.4ml
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab287848 has not yet been referenced specifically in any publications.