Product Details
| abID | ab234049 |
|---|---|
| Cat # +Size | K338-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em=535/587 nm) |
| Applications | Measurement of NADH in various tissues/cells Measurement of low levels of NADH in enzymatic reactions Analysis of metabolism and cell signaling in various cells |
| Features & Benefits | • Highly sensitive method to detect low levels of NADH. • Simple, fast and convenient. • This assay can measure less than 8 nM NADH in a variety of samples. |
| Kit Components | • NADH Extraction Buffer • NADH Cycling Buffer • PicoProbe™ (DMSO) • NAD Cycling Enzyme Mix (Lyophilized) • NADH Standard (Lyophilized) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
NAD and NADH exist in all living organisms and are important for energy transformation and maintenance of the redox state of cells or tissues. The reduced form, NADH is a driving force for cells and plays a critical role in cell regulation and repair processes. It is of increasing interest to be able to measure low level of NADH in samples or in enzymatic reactions. BioVision’s PicoProbe™ NADH Assay kit provides a highly sensitive method to detect low level of NADH (NAD is not detected in the reaction). There is no requirement to purify NADH from samples. The NADH Recycling Enzyme Mix specifically recognizes NADH (not NADPH) in the enzyme recycling reaction. The assay is rapid, simple and can measure less than 8 nM NADH in a variety of samples.
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Can Ferricyanide interfere with the kit?
Yes, it can interfere with the assay at a concentration >0.01mM.
Can the kit work with RBC?
For quantifying NAD/NADH in RBC, the customer would need to collect RBC, then extract the cellular contents with the extraction buffer and proceed according to the protocol. We have not tested this kit with RBC specifically but there should not be any special requirement for this sample type. Deproteinization by filtering the RBC extract through a 10KDa filter is recommended to remove all proteins/enzymes which can consume NAD/NADH as cofactors.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Tsvetkov et al., NADH Binds and Stabilizes the 26S Proteasomes Independent of ATP. J. Biol. Chem., Apr 2014; 289: 11272 - 11281.
For more citations of this product click here
