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PicoProbe™ Lactate Fluorometric Assay Kit

based on 1 citations in multiple journalsPicoProbe™ Lactate Fluorometric Assay Kit14.1 4
The most Sensitive Kit on the market, HTS
Catalog #: K638

Product Details

Cat # +Size K638-100
Size 100 assays
Detection Method Fluorescence (Ex/Em= 535/587 nm)
Applications The assay can detect L(+)-lactate less than 0.2 µM in a variety of biological samples. Analysis of metabolism and cell signaling. Mechanistic study for diabetes.
Features & Benefits • Simple, rapid, and high-throughput adaptable.
• Kit is the most sensitive Lactate Assay kit on the market.
Kit Components • Lactate Assay Buffer
• PicoProbe™ (in DMSO)
• Lactate Enzyme Mix (lyophilized)
• Lactate Substrate Mix (lyophilized)
• L(+)-Lactate Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Lactate (CH3CH(OH)COO-) plays an important role in many biological processes. Abnormally high concentrations of lactate have been related to diseases such as diabetes, lactic acidosis, etc. L(+)-Lactate is present in blood and is the major lactate stereoisomer formed in human intermediary metabolism. D-Lactate is also present but only at about 1-5% of L(+)-Lactate concentration. Lactate is a significant energy source for living organisms and can be used to generate cellular ATP. BioVision’s PicoProbe™ Lactate Assay kit is suitable for measuring very low levels of L(+)-lactate in a variety of samples. In this assay, L(+)-lactate is specifically oxidized to form an intermediate that reacts with a colorless probe to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to the amount of lactate. This simple, rapid & high-throughput suitable assay kit is the most sensitive Lactate Assay kit on the market. It can detect L(+)-lactate less than 0.2 µM in a variety of biological samples.

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The fluorescence counts are very low (less than 100) in the standards. Samples show higher counts (100-300) but when the amount of samples is doubled, the fluorescence intensity is not doubled. What could have gone wrong?
The assay buffer and the probe need to be warmed to RT or 37C before the assay. This is essential for the reaction to proceed at its normal pace. If either components are not warmed up enough, the reaction is slow and after 30 mins of incubation, you could get low readings and readings that are not linearly correlated. There is a lag time in this case when the enzyme activity ramps up. You could incubate longer, but that would not result in the highest possible readings. Warming up these two components, mixing them well and spinning down to make sure nothing is stuck to the cap and then proceeding with the assay should help. Adding more samples might have led to saturation and hence RFU values do not double upon adding twice the amount of sample.
Will carrying out the reaction at 37C help instead of RT?
Our recommended incubation temperature is RT (25C).It is not recommended to increasing it to 37C. The enzymes in the assay might show different kinetics at 37C than what was used to develop and optimize the assay. Some enzymes slow down at sub-optimal temperatures.
Can the samples be stored for a few days at 4C?
We do not recommend storing samples at 4C. Lactate can be very quickly degraded by enzymes like LDH in the sample. Hence using a spin filter column like Cat#1997 is a quick way of deproteinizing samples. Following this, samples can be stored at -80C in aliquots to be assayed at a later time.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?c
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Is it ok to use the K668 assay buffer for K638 or vice versa?
We highly recommend using the Assay Buffer provided with each kit for sample preparation for that particular assay. However, the composition of the Assay Buffer for K668-100 and K638-100 are the same and so you can use one of them for both the assays.
Wang et al., JMJD5 regulates PKM2 nuclear translocation and reprograms HIF-1α–mediated glucose metabolism. PNAS, Dec 2013; 10.1073/pnas.1311249111.
For more citations of this product click here