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PicoProbe™ Hypoxanthine Phosphoribosyl Transferase Activity Assay Kit (Fluorometric)

Highly Sensitive Assay for measuring HPRT
Catalog #: K478
$475.00

Product Details

Cat # +Size K478-100
Size 100 assays
Kit Summary • Detection method- Fluorometric (Ex/Em 535/587 nm)
• Applications- Measurement of Hypoxanthine Phosphoribosyl Transferase activity in purified/crude enzyme/ Cell lysate/ Tissue Lysate
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Eukaryotes
Applications Measurement of Hypoxanthine Phosphoribosyl Transferase activity in purified/crude enzyme/cell lysate/tissue lysate preparations
Features & Benefits • Simple, rapid & convenient assay to measure HPRTActivity in purified enzyme
• Includes Positive Control
Kit Components • HPRT Assay Buffer
• HPRT Substrate I
• HPRT Substrate II
• HPRT Developer
• HPRT Enzyme Mix
• HPRT Probe
• IMP Standard
• HPRT Positive Control

Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Hypoxanthine Phosphoribosyl Transferase or HPRT (EC 2.4.2.8) plays an important role in the generation of purine nucleotides through the purine salvage pathway and is present in prokaryotes and eukaryotes. The enzyme converts guanine to guanosine monophosphate and hypoxanthine to inosine monophosphate by transferring a phosphoribosyl group from phosphoribosyl pyrophosphate (PRPP) to the purine molecule, and releases an inorganic pyrophosphate molecule in the process. Mutations in HPRT lead to developmental disorders such as Lesch Nyhan Disease (LND), characterized by neurological and behavioral abnormalities. Its deficiency also leads to defective basal ganglia expression of the neurotransmitter dopamine and aberrant neuronal function. This causes dysregulation of multiple dopamine- related developmental functions and cellular signaling defects. BioVision’s HPRT Activity Assay Kit is a simple one-step plate based assay kit for the measurement of HPRT activity in biological samples. HPRT catalyzes the conversion of hypoxanthine to inosine monophosphate (IMP). IMP then undergoes a series of enzymatic reactions to convert a non-fluorescent probe to a fluorescent product. The signal, which is proportional to the generated product can be read in kinetic mode at Ex/Em 535/587 nm. The assay can detect as low as 2 mU HPRT.


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