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PicoProbe™ Glycerol-3-Phosphate Assay Kit (Fluorometric)

Highly Sensitive; Includes Paraoxonase Positive Control
Catalog #: K196

In stock

$455.00

Product Details

Cat # +Size K196-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (535/587 nm)
• Applications:
-Measurement of Glycerol-3-Phosphate in various tissues/cells
-Analysis of carbohydrate and lipid metabolism and cell signaling
-Drug screening
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Eukaryotes
Applications • Applications:
-Measurement of Glycerol-3-Phosphate in various tissues/cells
-Analysis of carbohydrate and lipid metabolism and cell signaling
-Drug screening
Features & Benefits • Simple, sensitive, and rapid.
• This assay kit can detect G3P less than 20 pmol/well and can be used for a variety of sample types.
Kit Components • G3P Assay Buffer
• G3P Enzyme Mix
• G3P Developer
• PicoProbeTM (in DMSO)
• G3P Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glycerol-3-phosphate (G3P) is an important intermediate for all living organisms. Glycerol-3-Phosphate is produced either by glycerol via glycerol kinase or by glycerol-3-phosphate dehydrogenase from dihydroxyacetone phosphate. In response to cellular signals, glycerol-3-phosphate can be utilized in multiple pathways: it can be further converted into glyceraldehyde-3-phosphate and enter glycolysis, rapidly generate NAD+ in brain or muscle tissues through the G3P shuttle, or enter the lipid biosynthetic pathway. Recent studies have found that glycerol-3-phosphate is a novel regulator and plays a fundamental defense role in plant pathogenesis. BioVision’s PicoProbe™ Glycerol-3- Phosphate Assay Kit is a sensitive, fast and easy-to-use method of measuring G3P concentration in various biological samples. In this assay, G3P is converted into an intermediate by the G3P Enzyme Mix. The G3P developer then utilizes the intermediate to generate a fluorometric signal with PicoProbe™, which can be used to calculate the concentration of G3P in the sample. This assay kit can detect G3P as little as 20 pmol/well and can be used for a variety of sample types


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