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PicoProbe™ Glutamate Assay Kit (Fluorometric)

Catalog #: K413

In stock

$455.00

Product Details

Cat # +Size K413-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (535/587 nm)
• Applications-Detects glutamate amounts as low as 5 pmol.
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Eukaryotes
Applications Measurement of Glutamate in various biological samples/preparations
Features & Benefits • Simple procedure; takes ~ 60 minutes
• Fast and convenient
• The kit provides an accurate assay for measuring inositol in various samples
Kit Components • Glutamate Assay Buffer
• PicoProbe™ (in DMSO)
• Glutamate Enzyme Mix
• Glutamate Substrate Mix
• Glutamate Standard (0.1 M)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glutamate (Glutamic Acid, Glu) is one of the most abundant proteogenic amino acids in humans. It is a key molecule in cellular metabolism. For example, Glutamate plays an important role both in the metabolism of some amino acids and disposal of excess nitrogen in the form of urea. In addition, Glutamate is the main excitatory neurotransmitter in mammalian nervous system and involved in learning, cognitive and memory processes. Glutamate signaling activates receptors which have been implicated in mental disorders (Schizophrenia), neurodevelopment disorders (autism) and Neurodegenerative diseases like Alzheimer’s and Parkinson’s disease. BioVision’s PicoProbeTM Glutamate Assay Kit (Fluorometric) allows for quantification of Glutamate in Biological Fluids and Tissues. The assay is based on an enzymatic reaction in which a fluorogenic probe is reduced producing a stable signal. The reduced fluorophore produces a strong signal (Ex/Em= 535/587 nm), which is directly proportional to the amount of Glutamate in samples. The assay is simple, reproducible, and can specifically detect as low as 5 pmol of Glutamate in a 100 µl reaction


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