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Product Details
| Cat # +Size | K688-100 |
|---|---|
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em 535/587 nm) |
| Applications | This assay kit can detect less than 0.5 µM glucose in various biological samples. Analysis of metabolism and cell signaling. Mechanistic study of obesity and diabetes. |
| Features & Benefits | • Simple procedure • Fast and convenient • The assay is ultra-sensitive and suitable for high-throughput |
| Kit Components | • Glucose Assay Buffer • PicoProbe™ (in DMSO) • Glucose Enzyme Mix (lyophilized) • Glucose Substrate Mix (lyophilized) • Glucose Standard (100 mM) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Glucose is the main energy source for virtually all living organisms. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes. Biovision’s PicoProbe™ Glucose Assay kit is simple, rapid, ultra-sensitive and suitable for high-throughput. In this assay, D-glucose is enzymatically oxidized to form a product which reacts with a colorless probe to generate the fluorescence (Ex/Em = 535/587). The fluorescence generated is directly proportional to the amount of glucose. This assay kit can detect less than 0.5 µM glucose in various biological samples.
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What are the difference between the K606, K686 and K688 assay kit?
There are many differences between these 3 kits although they all measure the sample glucose concentration. A few of these differences which might make your decision of making a choice easier are:K606-100:Applicable for both colorimetric and fluorimetric detection.Requires filters for 570 nm and Ex/Em: 535/587 nm.Detetion limit of 1-10000 uM glucoseUtilizes the oxired probeK686-100Applicable only for colorimetric detection.Requires filters for 450 nm.Detetion limit of 20 uM to 10 mM glucoseDoes not utilize Oxired or Picoprobe (TM)Ideal for samples having reducing substancesK688-100Applicable only for fluorimetric detection.Requires filters for Ex/Em: 535/587 nm.Detetion limit of < 0.5 uM glucoseUtilizes the Picoprobe (TM)
Can this kit be used with samples like bacteria, plants, drosophila etc?
We have optimized the kit with mammalian samples. However, theoretically these kits should work with samples from multiple species/sources. Since the optimal conditions depend on the sample type, the protocol has to be be adapted to fit the samples for efficient results. Please refer to this kit's citations to see what kind of samples have been used with this kit other than mammalian samples.
Can we use frozen samples with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Can we use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor."
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Why are my standard curve values lower than those shown on the datasheet?
There are multiple factors which influence the signals like the incubation times, room temperature, handling etc. In general, to increase the value of the standards, you can increase the incubation time. As long as the standard curve is linear, it should be fine to use, since all of your samples will also be measured under the same conditions on this curve.
How do I normalize my samples against protein concentration
You can use a protein quantitation assay on the supernatants you get from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Should I make a standard curve for every expt I do, or is one curve/kit enough?
Yes, I would strongly recommend you to do the standards every time you do the expt. There is always a chance that something was done differently that day and we do not want any conditions to differ between standards and samples.
Jiménez-Amilburu et al., GLUT12 deficiency during early development results in heart failure and a diabetic phenotype in zebrafish. J. Endocrinol., Jan 2015; 224: 1 - 15.
Shih et al., Flavin containing monooxygenase 3 exerts broad effects on glucose and lipid metabolism and atherosclerosis. J. Lipid Res., Jan 2015; 56: 22 - 37.
For more citations of this product click here
