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PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)

The most sensitive kit on the market
Catalog #: K751
$525.00

Product Details

Cat # +Size K751-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 535/587 nm)
Applications Measurement of G6PDH activity in various tissues and cells
Evaluation of pentose phosphate pathway
Features & Benefits • Rapid & simple
• High-throughput adaptable
• Can detect as low as 1 µU of G6PDH activity
Kit Components • G6PDH Assay Buffer
• PicoProbe™ (in DMSO)
• G6PDH Substrate
• G6PDH Developer
• G6PDH Positive Control
• NADPH Standard (200 nmol)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glucose-6-Phosphate Dehydrogenase (G6PDH: EC 1.1.1.49) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as liver, mammary gland, adipose tissue, and adrenal gland. BioVision’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Assay kit provides a quick and easy method for monitoring G6PDH activity in a wide variety of samples. In this assay, G6PDH converts G6P into pyruvate and NADPH, which further reduces PicoProbe™ to generate an intense fluorescence product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 1 µU of G6PDH activity.


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Can we use frozen samples with this assay?
We would recommend using fresh samples. However, frozen samples can be used too provided they have not undergone multiple freeze thaw cycles.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6-12 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.