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PicoProbe™ Fructose Fluorometric Assay Kit

Highly Sensitive Assay, HTS
Catalog #: K611

In stock

$425.00

Product Details

Cat # +Size K611-100
Size 100 assays
Detection Method Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Mammalian
Applications The kit detects fructose in the range of 5 to 500 picomoles/well.
Features & Benefits • Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of fructose concentrations in biological samples
Kit Components • PicoProbe Fructose Assay Buffer
• PicoProbe
• Sample Cleanup Mix
• Conversion Enzyme
• Fructose Enzyme Mix
• Fructose Substrate Mix
• Fructose Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Fructose is a monosaccharide found in many foods and is one of the three most important blood sugars along with glucose and galactose. Fructose is the sweetest naturally occurring sugar, estimated to be twice as sweet as sucrose. In BioVision’s PicoProbe™ Fructose Assay Kit, free fructose is enzymatically processed with the formation of a metabolite which reacts with the PicoProbe to generate fluorescence (Ex/Em = 535/587 nm). The kit provides a simple, highly sensitive, reliable method suitable for high throughput assay of D-fructose. Glucose interference can be removed by using the Sample Cleanup Mix. The PicoProbe™ Fructose Assay Kit can detect fructose in the range of 5 to 500 picomoles/well.


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What is the difference between K611 and K619 (Fructose colorimetric/fluorometric assay kit)?
The picoprobe allows K611 to be manifold more sensitive than the K619. K611 detects fructose in the range of 5 to 500 picomoles/well. Using K619 fluorometric method, Fructose concentration in nmoles/well can be measured.
The values of the std curve are in the low hundreds. Why?
It is important to warm up the probe to >20C and the assay bufefr to RT to ensure that the reaction proceeds according to the normal time of incubation. Incompletely thawed or cold reagents can lead to a slow reaction and low values.
Is deproteinizing recommended for all samples?
Deproteinizing helps remove proteins/enzymes which can use up Fructose. This is especially important for highly metabolically active tissues. For best results, it is recommended to deproteinize all samples inclusing serum, cell lysates etc.
Does the amount of probe need optimization for every experiment?
The probe amount should be optimized for specific sample types using a few representative samples and standards to ensure quenching or high background are not affecting the results. The gain/sensitivity of the fluorometer should be set to medium or low to avoid amplifying signal from noise.
If the sample clean-up mix is used, is it necessary to multiply by a dilution factor at the end?
Yes, it is recommended that the final calculations be multiplied by a dilution factor. However, if all samples are treated with 1ul of sample clean-up mix, it is acceptable to omit a dilution factor since all samples get equally diluted by this addition.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
How are samples normalized against protein concentration?
A protein quantitation assay can be used with the supernatants from cell/tissue lysates or with any other liquid sample in the assay buffer.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can the assay buffer from K619 be used with this kit?
We do not recommend swapping the buffers since optimal conditions (pH, salts etc.) for the specific assay are provided by the assay buffer.