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PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric)

Rapid, sensitive & simple assay to measures D-2Hydroxyglutarate Dehydrogenase activity
Catalog #: K248
$495.00

Product Details

Cat # +Size K248-100
Size 100 assasys
Detection Method Fluorescence (Ex/Em=535/587 nm)
Species Reactivity Mammalian
Applications Detection of D-2-Hydroxyglutarate Dehydrogenase activity
Features & Benefits • Rapid, sensitive and convenient
• The kit measures total Activity of D-2-Hydroxyglutatare Dehydrogenase Activity with limit of quantification 4 µU/µl using recombinant D2HGDH
Kit Components • D2HGDH Assay Buffer
• PicoProbe™ (in DMSO)
• D2HGDH Substrate
• D2HGDH Developer
• D2HGDH Positive Control
• NADH Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH: EC 1.1.99.2) is a mitochondrial enzyme which catalyzes the conversion of D-2-Hydroxyglutarate into 2-Oxoglutarate. In humans, mutation of D2HGDH gene could reduce D2HGDH activity which will cause the D-2-Hydroxyglutarate accumulation in body fluids. Increased D-2-Hydroxyglutarate level in body fluids leads to D-2-Hydroxyglutaric Aciduria, a neurometabolic disorder characterized as developmental delay, epilepsy, hypotonia, and dysmorphic features. BioVision’s PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay kit provides a quick and easy method for monitoring D2HGDH activity in a wide variety of samples. In this assay, D2HGDH converts D-2-Hydroxyglutarate into 2-Oxoglutarate and NADH, which further reduces PicoProbe™ to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, sensitive and high-throughput adaptable and can detect as low as 4 µU/µl of D2HGDH activity.


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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.