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PicoProbe™ ADP Assay Kit (Fluorometric)

Fast, sensitive, HTP compatible, fluorometric ADP assay with detection limit as low as 10 pmol or 0.05 µM of ADP.
Catalog #: K211

In stock

$455.00

Product Details

Cat # +Size K211-100
Size 100 assays
Detection Method Fluorometric analysis (Ex/Em = 535/587 nm)
Species Reactivity Mammalian
Applications Measurement of ADP in various tissues/cells
Analysis of metabolism and cell signaling in various cell types
Features & Benefits • Ultrasensitive, Simple, HTP adaptable fluoromotericassay kit for ADP measurement.
• Can detect as low as 10 pmol or 0.05 µM of ADP
• Detection method- Fluorometric analysis (Ex/Em = 535/587 nm)
• Applications- Detection of NOS activity
• Sample Type: Tissue or cell extracts
Kit Components • ADP Assay Buffer
• ADP Enzyme Mix I
• ADP Enzyme Mix II
• ADP Developer
• PicoProbe™ (in DMSO)
• ADP Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Adenosine diphosphate (ADP) is an organic molecule that plays a critical role in cellular metabolism and energy transfer reactions. ADP is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. These dephosphorylations and rephosphorylations occur via various phosphorylases and kinases. ADP is stored in platelets and can be released to interact with variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes also occur in the cytoplasm. Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is generally not available in most laboratories. BioVision’s PicoProbe™ ADP Assay Kit provides a sensitive fluorescence method to detect ADP. The kit is suitable for measuring ADP levels in various samples, including the ones that contain reducing substances, which may interfere with oxidase-based assays. In this assay, ADP, in the presence of ADP Enzyme Mix is converted to an intermediate, which reacts with the fluorescent probe to generate a strong stable signal at Ex/Em = 535/587 nm. BioVision’s PicoProbe™ ADP Assay kit provides an ultrasensitive, fast and high-throughput ready assay for detection of as low as 10 pmol or 0.05 µM of ADP in samples.


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Can I use Perchloric acid to deproteinize my samples?
We strongly recommend using the 10Kda spin column. Perchloric acid needs to be neutralized completely before proceeding with the assay.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.