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Phospholipase C Activity Assay Kit (Colorimetric)

To determine the PLC activity
Catalog #: K2016
$495.00

Product Details

Cat # +Size K2016-100
Size 100 assays
Kit Summary BioVision’s Phospholipase C Activity Assay Kit is a direct assay to determine the PLC activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity. The assay is rapid, sensitive and is a convenient tool for detecting PLC activity. It can detect as low as 0.1 mU PLC activity under the assay conditions.
Detection Method Absorbance, OD 405 nm
Species Reactivity All
Applications Detection of PLC activity in various samples
Features & Benefits • A direct assay to determine the PLC activity in various biological samples.
• A convenient tool for detecting PLC activity.
• Sensitive
• Rapid
Kit Components PLC Assay Buffer
PLC Substrate
PLC Enzyme
PLC Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phospholipase C (PLC) belongs to a family of membrane-associated enzyme that specifically cleaves the phosphoester linkage before the phosphate group in phospholipids. PLC catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) into inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG), which are second messengers critical for downstream signal pathways to control diverse cellular processes. PLC family consists of 13 isozymes split between six subfamilies. Members of the PLC family are involved in intra and inter cellular signal transduction pathways that are important for cell proliferation and differentiation. PLC isozymes have been also associated with several diseases such as cancer, inflammation, cardiovascular diseases and neuropathic pain. Therefore, investigation of PLC enzymes and their inhibition may lead to innovative therapeutic strategies. BioVision’s Phospholipase C Activity Assay Kit is a direct assay to determine the PLC activity in various biological samples. The assay uses a specific PLC chromogenic substrate to detect PLC activity and the generated final product can be measured colorimetrically at OD 405 nm. The OD signal is proportional to the PLC activity. The assay is rapid, sensitive and is a convenient tool for detecting PLC activity. It can detect as low as 0.1 mU PLC activity under the assay conditions.


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