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Phosphoglycerate Mutase Activity Assay Kit (Colorimetric/Fluorometric)

to detect PGAM activity in biological samples
Catalog #: K2007
$495.00

Product Details

Cat # +Size K2007-100
Size 100 assays
Kit Summary Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3‐phosphoglycerate to 2‐phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target. BioVision’s PGAM Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate, which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with an OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.
Detection Method Absorbance (OD 570 nm) or Fluorescence (Ex/Em = 535/587 nm)
Species Reactivity All
Applications • Analysis of glycolytic metabolism in different sample types
• Mechanistic studies of various cancers
• Screening anti-cancer drugs
• Measurement of PGAM activity in various tissues/cells
Features & Benefits • Rapid
• Sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.
• Simple
Kit Components PGAM Assay Buffer
PGAM Positive Control
OxiRed™ Probe
PGAM Developer
2-PG Standard
PGAM Converter 1
PGAM Converter 2
PGAM Substrate
PGAM Lysis Buffer
PGAM Cofactor
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phosphoglycerate Mutase (PGAM) is an important enzyme in the glycolytic pathway that catalyzes the reversible conversion of 3‐phosphoglycerate to 2‐phosphoglycerate. It plays an important role in regulating glycolysis and anabolic activity to promote cancer cell proliferation. Several studies reported that PGAM is upregulated in many human cancers. Inhibition of this enzyme could lead to tumor cell death thereby making PGAM an attractive therapeutic target. BioVision’s PGAM Activity Assay Kit can be used to detect its activity in biological samples. The kit utilizes the ability of an active PGAM to catalyze the conversion of 3-Phosphoglycerate to 2-Phosphoglycerate, which is subsequently used to generate an intermediate product. The intermediate product then stoichiometrically reacts with an OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This assay kit provides a rapid, simple and sensitive method to detect PGAM activity as low as 0.05 mU in a variety of samples.


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