Product Details
| abID | ab252890 |
|---|---|
| Cat # +Size | K194-100 |
| Size | 100 assays |
| Detection Method | Colorimetric (OD = 340 nm) |
| Species Reactivity | All |
| Applications | Mechanistic study for cancer development: e.g. Gastric Cancer,Measurement of Phosphoglycerate Kinase (PGK) Activity in various tissues/cells.,Analysis of glycolytic Pathway and Gluconeogenesis Pathway |
| Features & Benefits | • Sensitive (~ 50 mU),Fast & Convenient,Protocol takes ~60 minute to be completed,Simple procedure |
| Kit Components | • PGK Substrate • NADH • PGK Developer • U.V. Transparent Plate (96-well) • ATP • PGK Positive Control • PGK Assay Buffer |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Phosphoglycerate Kinase (PGK1) (EC 2.7.2.3) participates in two of the most important biochemical pathways in living organisms: Glycolysis and Gluconeogenesis. PGK is responsible for the catalysis of the reversible reaction of 1,3-Bisphosphoglycerate (1,3-BPG) and ADP to 3-Phosphoglycerate (3-PG) and ATP. In humans, PGK1 and PGK2, two PGK isozymes, have been characterized. While PGK1 is X-Chromosome-linked variant and expressed in all cells, PGK2 is autosome-linked variant and uniquely expressed in spermatogenic cells. Recent studies have shown deficiency of Phosphoglycerate Kinase could lead to chronic hemolytic anemia, mental disorders and myopathy in humans, whereas PGK overexpression promotes gastric cancer cell invasiveness. Therefore, accurate measurement of Phosphoglycerate Kinase Activity is useful for mechanistic and possible diagnostic studies. BioVision’s PGK Activity Assay kit provides a quick and easy way for monitoring PGK activity in various samples. In the first step of this enzymatic assay, PGK converts 3-Phosphoglycerate and ATP to 1,3-Bisphosphoglycerate and ADP. The nascent intermediate is detected via a series of enzymatic reactions that lead the oxidation of NADH to NAD+, which can be easily detected.
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