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Phosphoenolpyruvate Carboxylase Activity Assay Kit (Colorimetric)

Simple, sensitive, enzymatic assay to measurePhosphoenolpyruvate carboxylase activity in biological samples. Only kit in the market including activie positive control
Catalog #: K443

In stock

$455.00

Product Details

Cat # +Size K443-100
Size 100 assays
Kit Summary • Detection method - Colorimetric (OD 570 nm)
• Species reactivity - Mammalians
• Applications - Measurement of Phosphoenolpyruvate carboxylase activity in various biological samples, such as plants, bacteria and purified protein
• Includes active PPC
Detection Method Colorimetric (Absorbance 570 nm)
Species Reactivity Bacterial/Plants
Applications • Measurement of Phosphoenolpyruvate carboxylase activity in various biological samples and protein preparations
Features & Benefits • Simple, highly sensitive, high-throughput compatible
Kit Components • PPC Assay Buffer
• PPC Substrate A
• PPC Substrate B
• PPC Probe (in DMSO)
• PPC Enzyme Mix
• PPC Standard (100 mM)
• PPC Positive Control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phosphoenolpyruvate carboxylase (EC 4.1.1.31, PPC) is an enzyme found in plants and some types of bacteria. It catalyzes the formation of oxaloacetate from phosphoenolpyruvate by fixing one carbon in the forms of either carbon dioxide or bicarbonate. PPC participates in carbon fixation in C4 photosynthesis and Crassulacean acid metabolism (CAM) plants and acts as an anaplerotic enzyme, which replenishes TCA cycle intermediates, in bacteria and C3 plants. The participation of PPC in the carbon fixation cycle of C4 plants enable them to survive in high sunlight but dry environments. BioVision’s Phosphoenolpyruvate Carboxylase Assay Kit provides a quick and easy method for the measurement of PPC activity in various samples. In this assay, Phosphoenolpyruvate Carboxylase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converter generating a colorimetric signal (OD 570 nm). The generated absorbance signal is directly proportional to the amount of active PPC present in the samples. This kit provides a simple, fast and high throughput adaptable assay to measure the activities of PPC as low as 0.2 mU


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