Phospho-Raf Antibody

Catalog #: 3504 | abID:

Product Details

Cat # +Size 3504-100
Size 100 µg
Antibody Target Phospho-Raf (THR259)
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Synthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf
Accession # P04049
Gene ID 5894
Appearance Colorless liquid
Concentration 0.5 mg/ml
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-Phospho-Raf (Ser259) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification Affinity purified
Species Reactivity Human, mouse, rat
Application Western blot, Immunohistochemistry
Application & Usage Western blotting (0.5-4 µg/ml) and Immunohistochemistry (20-40µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


c-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.

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