You have no items in your shopping cart.
Product Details
| Cat # +Size | 3269-100 |
|---|---|
| Size | 100 µg |
| Antibody Target | Bad |
| Host | Rabbit |
| Antibody Type | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser112 of mouse Bad |
| Accession # | Q61337.1 |
| Gene ID | 2493288 |
| Appearance | Colorless liquid |
| Concentration | 0.2 mg/ml |
| Formulation | 100 µg (0.2 mg/ml) peptide affinity purified rabbit anti-Phospho-Bad (Ser112) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal. |
| Purification | Affinity purified |
| Species Reactivity | Human, mouse, rat |
| Application | Western blot, Immunoprecipitation, Immunohistochemistry |
| Application & Usage | Western blot analysis (1-2 µg/ml) and immunoprecipitation (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112. |
| Handling | The antibody solution should be gently mixed before use. |
| Storage Conditions | -20 °C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.
Why buy BioVision Products?
 |
 |
 |
 |
Innovation |
Affordability |
Global Presence |
Technical Support |
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
BioVision is committed to providing the highest quality products at a competitive price. |
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery. |
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently. |
