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Phospho-Akt Antibody

based on 6 citations in multiple journalsPhospho-Akt Antibody64.1 4
Catalog #: 3257

Product Details

Cat # +Size 3257-100
Size 100 µg
Antibody Target Phospho-Akt
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Synthetic phospho-peptide corresponding to residues surrounding Ser473 of mouse Akt
Accession # P31749
Gene ID 60391226
Appearance Colorless liquid
Concentration 0.5 mg/ml
Formulation 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Phopho-Akt (Ser473) polyclonal antibody in phosphate buffered saline (PBS), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification Affinity purified
Species Reactivity Human, mouse, rat
Application Western blot, Immunoprecipitation
Application & Usage Western blotting (0.5-4 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The phospho-Akt (Ser473) antibody detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Akt, also referred to as PKB and RAC, is the cellular homologue of the viral oncogene v-Akt. This protein kinase is activated by insulin and various growth factors and functions in a pathway involving PI3 kinase as activation is sensitive to wortmanin. Recent evidence sμggests that Akt functions to promote cell survival by actively inhibiting apoptosis, althoμgh the pathways involved have not been identified. Akt is activated by phospholipid binding and phosphorylation within the activation loop at Thr308 and within the C-terminus at Ser473.

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Zeyda, M. et. al. Osteopontin Is an Activator of Human Adipose Tissue Macrophages and Directly Affects Adipocyte Function. Endocrinology, Jun 2011; 152: 2219 - 2227.
Braydich-Stolle, L. et al. Silver Nanoparticles Disrupt GDNF/Fyn Kinase Signaling in Spermatogonial Stem Cells. Toxicol. Sci., 2010; 116: 577-589.
Stadbauer K et al (2009) Am J Physiol Endocrinol Metab; 10.1152/ajpendo.00061.2009.
Artwohl M et al. (2007) Am. J. Physiol. Endocrinol. Metab. 293: E681-E689.
Saemann, M.D., et al. (2005) J. Clin. Invest. 115: 468-475.
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