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Phosphatidylserine Assay Kit (Fluorometric)

The first kit in the market to measure Phosphatidylserine in samples
Catalog #: K565
$495.00

Product Details

Cat # +Size K565-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em= 535/587 nm)
• Sample type- cell and/or tissue lipid extracts
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and non-ELISA alternative to detect Phosphatidylserine amount in biological samples.
Detection Method Fluorescence (Ex/Em= 535/587 nm)
Species Reactivity Eukaryotes
Applications This assay provides a convenient and non-ELISA alternative to detect Phosphatidylserine amount in biological samples.
Features & Benefits Simple, fast, does not require lenghty incubation times
Kit Components • Phosphatidylserine Assay Buffer
• Probe Solution
• Lipase Enzyme Mix
• Serine Enzyme Mix
• Developer Enzyme Mix
• Phosphatidylserine Standard (1 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phosphatidylserine (PS) is a glycerophospholipid consisting of a phosphatidyl group attached to L-serine via a phosphodiester linkage. PS is a critical component of the cellular plasma membrane and accounts for 2-15% of plasma membrane lipid composition, depending on the cell/tissue type. The highest concentrations of PS are found in neuronal tissues, where it is critical for maintaining conduction velocity in myelinated neurons, as well as for higher order cognitive skills such as learning and memory. In normal, healthy cells, PS is held in the inner membrane surface (facing the cytosol) by the lipid transporter protein flippase. However, in apoptotic cells, PS molecules ‘shuffle’ between the inner and outer plasma membrane monolayers. When PS molecules flip to the extracellular (outer) surface of the cell membrane, they act as a signal for macrophages to engulf and digest the (apoptotic) cell. BioVision’s Phosphatidylserine Assay Kit allows for quantification of PS in lipid extracts of cell/tissue lysates. The assay is based on the enzymatic cleavage of PS to yield phosphatidic acid and L-serine, which is subsequently metabolized and reacts with a probe to form a stable fluorophore (Ex/Em = 538/587 nm). The assay is selective for PS (other phospholipids such as phosphatidylcholine, phosphatidylethanolamine or phosphatidic acid do not interfere). The kit is high-throughput ready and highly sensitive (can detect as little as 50 pmole/well of PS (5 μM in a 10 μl sample volume)).


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